Related Manuals for BD Accuri C6 . endstream endobj 2781 0 obj <>stream The Accuri C6 cytometer in my lab has some problems, and we need some help. User Tracking Installer Icon .....80 Figure C-2. The software for Accuri C6 (CFlow Plus) is very user-friendly and efficient. 1 The standard configuration of the BD Accuri C6 Plus (as shipped) is “3-blue 1-red” with the 533/30 (formerly 530/30) filter in FL1, 585/40 in FL2, 670 LP in FL3, and 675/25 in FL4. BD Accuri C6 Software provides the following features: a. Tabbed views for collection, analysis, and statistics b. The BD Accuri™ C6 Plus personal flow cytometer is the newest generation of the BD Accuri™ platform. The DNA histograms produced by the BD Accuri C6 display excellent peak CVs, and this instrument, uniquely, can provide simultaneous linear estimates of nuclear DNA content spanning at least two orders of … y\L }����~*L�l�n}��� 7�x�m����X��gȱ0Z1J50 m��@� �D�1Hğ����H31�m� �� ���' L��� x������ PP9R�P������t�Y�a9D��� ` +9|%��; ��0^��O���-�&+�M�n����I��]�: ���bh��*��f��M�=�>�����}'$�� BD Accuri™ C6 Flow Cytometer Instruction Manual, serial number 2400 and higher; BD Accuri™ C6 Software User Guide; BD CSampler™ Accessory Installation Guide; BD CSampler™ Software User Guide With minor signs of normal wear and tear. The BD Accrui C6 Cytometer -The cytometer has 2 lasers: -Blue 488nm laser (3 colors) -Red 640 (1 color) -Unlike most cytometers you will encounter, the Accuri does not all you to manually adjust your voltage settings -Instead, the software uses a larger logarithmic … It also includes … NOTE: The BD Accuri CFlow Importer reads the FCS data directly from the BD Accuri CFlow file, so you do not have to export individual files. Optional filters can be used to increase resolution or to separate fluorochromes with overlapping signals, like GFP and YFP. Such wear includes scratches, scrapes, and general dirtiness. Peristaltic Pumps and Luer Locks, Figure 4-5. ... Deionised water is recommended in the C6 manual - try this and it may stop blockages. Since the C6 fluidics system is non-pressurized, any brand of sample tube that is 12 x 75 mm or smaller can be used. Came with note that it needs repair. endstream endobj 2780 0 obj <>stream ���r��n�Ypm8I_�À�W�"v����,,{�m��c__$�`�r݀��\NiC�{i\ �p=�ƫ�k.x���8�ႃ3��{a�> �6111111111111111��Ǧ�� Along with traditional forward and side scatter, the C6 is an ideal instrument for basic analytical cytometry. 2798 0 obj <>/Filter/FlateDecode/ID[<3B235E56AD4D884590D8BFA25210CF82><756487A21B96FC4983BB2B9C4996548C>]/Index[2774 31]/Info 2773 0 R/Length 99/Prev 1549724/Root 2775 0 R/Size 2805/Type/XRef/W[1 3 1]>>stream Works with current protocols and reagents: Accuri developed the C6 to use the same reagents you currently use on the market-leading cytometers. Proper Attachment of Fluidics Lines, Figure 4-1. BD Accuri C6: BMSB J538. Laboratory Equipment BD CSampler Installation Manual 2 pages. Click here to view the C6 software user guide. BD Accuri C6 Flow Cytometer Instrument Manual Bd Accuri C6 Pdf User Manuals. BD Accuri C6 Instrument Manual (31 pages) Flow Cytometer. Figure 1-1. A unique, non-pressurized, peristaltic pump system drives the fluidics. Update Driver Software - Cytometer, Successfully Updated Your Driver Software, Figure 5-5.The C6 Software Status Message Box - C6 Is Connected and Ready. DNA/Cell cycle analysis BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express ™ software and FlowJo ™ software. BD Accuri C6 Instrument Manual (31 pages), Figure 1-1. the BD Accuri C6 Flow Cytometer System, BD Accuri C6 Flow Cytometer Specifications, Figure 3-1. Operate the instrument as described in this Instruction Manual. Sliding Luer Lock Fittings Off of Pump Head, Figure 5-2. (In this example, the file is FCS Express Import Demo.c6.) 1.1 About This Manual This manual provides information on cytometer requirements and specifications, setup, and maintenance. Accuri C6 is an analyzer equipped with two lasers and 4 color detectors and it has capabilities similar to FACSCalibur. %%EOF Manuals and User Guides for BD Accuri C6. endstream endobj 2775 0 obj <>/Metadata 476 0 R/Pages 2772 0 R/StructTreeRoot 613 0 R/Type/Catalog>> endobj 2776 0 obj <>/MediaBox[0 0 612 792]/Parent 2772 0 R/Resources<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI]/XObject<>>>/Rotate 0/StructParents 0/Tabs/S/Type/Page>> endobj 2777 0 obj <>stream Flow cytometry within reach The analytical power and versatility of today’s laser-based flow cytometry systems have unlocked the mysteries of cell biology and empowered entirely new fields of research. Accuri C6 is a digital PC based bench top analyser, 2 laser system. ۟��@��7����O����vƁ`���OW�p�>����8\:�7k������������>q=���!�\�$/-dzCL��)��"�nÒ��2fVo.C��I�aj���~��`��-`v�T�͖(V�` �㆔����rHf ��d)R�7��V�� ר�Gˌ%b��j�Z��.�f>�Z�i-h�`*G-�mA�S,�1&;e,��R�h�Q�4 Lh&4��{�,a��T���.hݓ�t�D�4,�L��J�'�uI�(Y�22��(�t�O�+L�op���z�¢exy����w�c#s����{�u^�(�����H� For a more complete set of directions refer to the BD Accuri C6 Flow Cytometer Instrument Manual . 3. 2804 0 obj <>stream Available to trained users with online booking. Even more colors to choose from with selectable lasers and filter replacement. ?���[��? �e�I�c~w1���0 ��� The BD Accuri C6 Plus is an ideal benchtop platform for core immunophenotyping studies and is configured for rapid and accurate analysis of up to six parameters at the single-cell level. Accuri C6 consumables, manuals, etc. This manual provides information on cytometer requirements and specifications, setup, and maintenance. BD Accuri C6 with plate handler April 2016 This protocol contains instructions for setting up an Accuri C6 with plate handler. If the BD Accuri software displays the message Extra Start-up time needed due to cleaning or improper shut down it will take the instrument several more minutes for the instrument to get to the green-light ready state. endstream endobj startxref BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. Core Size/Flow Rate Combinations.....79 Figure C-1. Such counts are essential in many research and clinical applications, including enumerating leucocytes, B cells, T cells, and platelets in human blood, measuring of … Instructional video on the basics of using BYU's Accuri C6 cytometer. View online or download Bd Accuri C6 Instrument Manual Bd Accuri C6 Manuals | ManualsLib BD Accuri™ C6 Flow Cytometer Optical Filter Guide . Accuri C6 flow cytometer fits in any lab and is readily accessible to all researchers—experts and novices alike. Includes all accessories shown in pictures. The BD Accuri™ C6 is a personal flow cytometer that brings cell analysis within reach by being easy to use, simple to maintain, and affordable. BD Accuri C6 Software User Guide - Manual vi 7820095-01 Rev-0 Table B-1. BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. Accuri™ C6 Plus BD Biosciences. Update Driver Software - Cytometer, Second Dialog Box, Figure 5-4. BD Accuri C6 flow cytometer for the analysis of plant genome sizes. BD Accuri C6 Flow Cytometer Optics and Fluidics (Top View, Lid Open), Changing the Workstation Computer Settings for Windows ® 7, Figure 3-5. y`�B}�@h�K�v�f�)�*Z�6D�&d'��f�z-C�Zn����R73�nǦ3�-m\�v��tN��ƫ�Zij7��um�zc�&��݌�d7�.� ��L�q����5�H=�Y�pQAں΃��^̑�]#�26m�H-���2'd��5g�Z1GZ;��i�� 9kK:::�;+:8��;:��+::X�;*�,v ����ţ����A(� Select the BD Accuri CFlow file (.c6) that contains the data you want to view with FCS Express. The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. User's Guides and Manuals. Services available on the BD Accuri: Single or multi-color cellular analysis (phenotyping, intracellular staining, etc.) The instrument will begin a 5 minute auto start cycle. Install, update, and run anti-virus protection software on a regular basis. Item is sold AS IS. This item has been powered on, but no further testing. The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. The BD Accuri C6 flow cytometer (Figure 2) offers performance, simplicity, and affordability. So the choice should be addressed by the kind of flow experiments you plan to do. The unique fluidics system of the BD Accuri C6 Plus, driven by peristaltic pumps, allows it to determine sample volume and count cells rapidly, directly, and automatically from the software, eliminating laborious plate counts. It also includes common troubleshooting help and service information. Installation Dialog Box .....80 Figure C-3. If you will be using a different system, consult the appropriate protocol in our flow cytometry protocols for Multiplex assays page, or contact our Technical Support team at technical@abcam.com. Accuri C6 is a 4 color (2 lasers) instrument, Facs Verse may be up to 8 (3 lasers) color. x���MkA�����dI#��b�!�@K =�BH}JK���z,�;��i��w����+���n����s�ج�b;���H Accuri C6 is an "easy to use" flow cytometer which can be connected to an autosampler (48 and 96 well, and 24 tube rack, we do not have one). Additional laser configurations (2-blue 2-red, 4-blue) are available with the Selectable Lasers Module (Cat. Press the Power button on the BD Accuri C6 instrument face panel. Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. In addition, C6 has a flow metering system for automatic calculation of absolute cell counts. 2774 0 obj <> endobj Many thanks, Matt . ��qq��p�{|�wtxs BD Accuri C6 Software (CFlow Plus) controls the BD Accuri C6 flow cytometer system in order to acquire data, generate statistics, and analyze results. x���M��� `(-X�:��&�̑� r�p�N��S�F?�R4�S�^L=�� I��t�_�n@Q4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_`���=��@J�'� �������(�⍴?�aV(�S��'3*{�����f����߫�!cE��+MH4~������U1Qy+��R�L�Vj��\i@���|����f�M�ZY?�`��bJ�ܠD��Ke��@��T`�e���8�HQ�W�8�7�y����R�T���p�]����ޣ�y.� nW�$�����[�}�k�[j�-J�����[�C�+�gA-�G鳅������g��6v���, ����R�s%�Yu���f�y'��8l���N%. `� T���4 d*u�y�Dn�cK�u��A�aZC���_��q�� �#��� Please note: It is possible to measure some other fluorochromes using this setup. Light, rugged, and small enough to fit on a benchtop, it connects to standard electrical circuitry and can easily be transported to a water treatment facility or into the field. Overall very good cosmetic condition. For Research Use Only. Only operate the Accuri C6 Flow Cytometer with an original copyrighted version of software authorized by Accuri. h�bbd```b`` � ��9������"ɸD�NB��!�6�[��f����3>��������L�\�`��X��?�� �YF endstream endobj 2778 0 obj <>stream Standard fluorochrome in use are identical to FACS Calibur. h�b```d``ja`c``�� Ā B,@Q�G����^�M�*O�:.:�c�a��"~��!������Q���ȵ�۵8�1}p�l�����C�+x��L��a:_"�����.��r+��枘�! Accuri C6 consumables, manuals, etc. 21. Direct counts on the BD Accuri C6 Plus correlate highly with counting beads and are more precise than manual counts. 0 %PDF-1.6 %���� The BD Accuri™ C6 is a dual laser flow cytometer capable of detecting four distinct fluorochromes simultaneously. h޴S�j�0����2�X���d��BۇM�J��p�l�d�~5����adA�Ci���@�� No. This includes microfuge tubes and tubes made of polystyrene and polypropylene. A broad dynamic range of detection makes it easy to analyze cells as varied in size as platelets and eosinophils in the same data file. If you are interested in using a fluorochrome that is not listed here with the Accuri C6, please contact the operator.. Click here to view the instrument manual. BD Accuri™ C6 Compensation Calculator (Microsoft® Excel®) BD Accuri™ C6 Flow Cytometer Optical Filter Guide . �R'�B$K1�#��L�f����2Φ� 5gM�(�,��ycT�}�b��yL]��:y�y�:��~�Kw�s������b\Pע��._H�vtt哂n�mO*���n�s����;;�5����~��;;a��t��~�HX�ÛK We have 1 BD Accuri C6 manual available for free PDF download: Instrument Manual . !� �XB4��u*��yo7�5�(8.����.�����jo���U߅Ţ?=_��Jk X�3^��=8l�S����6ؽ�6]�w����Wߵ %��S&!bM\w��d�T�����&�B��~��s˟1U�R�l1V&�f�PL�L��K6�c 6�9� Automated Preventive Maintenance Notification Window, Figure 4-4. endstream endobj 2779 0 obj <>stream �! 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